Thermodynamic and Physical Analysis of the Multi-Stage Thermal Inactivation Processes of Kluyveromyces marxianus Inulinase

IRAQI JOURNAL OF APPLIED PHYSICS Vol. 22, No. 3, July-September 2026, pp. 453-456 Thermodynamic and Physical Analysis of the Multi-Stage Abdul Sattar J. Taha 1 Aseel M.I. Al-Mashahedah 2* Thermal Inactivation Processes of Kluyveromyces marxianus Inulinase 1... More

IRAQI JOURNAL OF APPLIED PHYSICS Vol. 22, No. 3, July-September 2026, pp. 453-456 Thermodynamic and Physical Analysis of the Multi-Stage Abdul Sattar J. Taha 1 Aseel M.I. Al-Mashahedah 2* Thermal Inactivation Processes of Kluyveromyces marxianus Inulinase 1 College of Health Medical Techniques, Al Bayan University, The thermal inactivation behavior of free inulinase derived from yeast Baghdad, IRAQ Kluyveromyces marxianus was examined using differential thermal analysis (DTA) 2 Department of Biology, over the temperature range of 30-80 °C. The thermograms revealed two distinct College of Education, endothermic transitions at 54 °C and 65 °C, indicating a multistage denaturation Al-Iraqia University, process. The initial transition corresponds to reversible local conformational Baghdad, IRAQ rearrangements, whereas the subsequent transition is associated with irreversible * Corresponding author email: unfolding and loss of catalytic activity. The observed thermal behavior is attributed Less