Distance Cumulative freq. 1.0 0.5 0.0075 0 Mito/bead amplitude ratio at 1 Hz 0.1236 1.0 DMSO 1.0 0.0745 NZ 2 µM at 1 Hz NZ 10 µM >0.9999 0.8637 >0.9999 0.8 LatA 50 nM Mito/bead amplitude ratio Effective stiffness (pN/µm) Effective stiffness (pN/µm) 0.6...
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Distance Cumulative freq. 1.0 0.5 0.0075 0 Mito/bead amplitude ratio at 1 Hz 0.1236 1.0 DMSO 1.0 0.0745 NZ 2 µM at 1 Hz NZ 10 µM >0.9999 0.8637 >0.9999 0.8 LatA 50 nM Mito/bead amplitude ratio Effective stiffness (pN/µm) Effective stiffness (pN/µm) 0.6 Ratio 250 0.0056 0.5 400 0.4 200 0.0016 0.8408 150 200 0.2 0.0 100 0.0 0 2 4 6 8 10 12 0 0.5 1.0 50 0 DMSO NZ 2 µM NZ 10 µM LatA 50 nM Distance to the bead (µm) Cumulative freq. 0 DMSO NZ 2 µM NZ 10 µM • • • • λ 150 DMSO 0.0161 LatA (10 nM) LatA (50 nM) <0.0001 400 100 G (Pa) 0.0195 0.0050 50 G (Pa) 200 4.0 0 0.0021 0 50 100 Average lifetime (ns) Mitochondria area fraction (%) 0.4692 <0.0001 • 3.5 Lat A Lat A 0 Spearman DMSO 10 nM 50 nM • r 0.024 0.75 0.89 µM µM SO nM nM 3.0 M 2 10 p-value 10 50 0.98 0.067 0.033 D o o A A oc oc t t N La La N The cytoplasmic shear modulus G is more correlated to the local density of mitochondria upon treatment with the highest concentration of Lat A. 2.5 DMSO NZ 2 µM NZ 10 µM labex
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