Small RNA Library Prep Kit for Illumina Platform Optimized for ultra-low RNA inputs Recommended Recommended RNA Specimen Amount volume and concentration Cell Lines 1,000 cells* Complete kits with reagents for all steps from 1,000 cells, 15 µL at 10 ng/µL to...
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Small RNA Library Prep Kit for Illumina Platform Optimized for ultra-low RNA inputs Recommended Recommended RNA Specimen Amount volume and concentration Cell Lines 1,000 cells* Complete kits with reagents for all steps from 1,000 cells, 15 µL at 10 ng/µL to Tissues 3’ ligation though size selection 0.5 mg* 200 ng/uL Blood 10 µL ** Includes all clean-up columns and reagents in one convenient kit – no need to purchase Plasma/Serum 200 µL several kits Urine 0.5 mL Tremendous time savings with size selection Various (Bodily Exosomes and fluid or cell-culture 15 µL at 50 pg/µL* without gels and lengthy elution steps EVs media) Enhancement of useful small RNA detection Saliva 0.2 mL (3-4x increase) in human plasma/serum by Min 4 x 10 µm sec- depleting an abundant sequence that may FFPE Sections tions of 1 cm² occupy up to 50% of NGS reads All concentrations measured by RiboGreen/Qubit. *Please note that these are Norgen Biotek’s recommended input volumes and concentrations to achieve optimal
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