Protein Expression Protocol & Troubleshooting In E Coli

In terms of recombinant expression, E. coli has always been the preferred microbial cell factory as it has multiple, significant benefits over other expression systems including cost, ease-of-use, and scale. Here, we present a general protocol of protein... More

In terms of recombinant expression, E. coli has always been the preferred microbial cell factory as it has multiple, significant benefits over other expression systems including cost, ease-of-use, and scale. Here, we present a general protocol of protein expression as well as a list of possible solutions when facing the challenge of expressing a new protein in E. coli. Less