How To Properly Set Up A 10-Color Flow Cytometry Experiment

In today’s world, a large number of flow cytometrists can access tools capable of experiments using 10 or even more colors. The step from two to ten colors might appear small, however there are various considerations in the structure and analysis of... More

In today’s world, a large number of flow cytometrists can access tools capable of experiments using 10 or even more colors. The step from two to ten colors might appear small, however there are various considerations in the structure and analysis of experiments which makes total use of tools which can handle these additional colors. Just imagine measuring a 2-color experiment. With Two biaxial plots plus a single quadrant gate, you end up with merely 4 populations to document. Next include a another color. By doing so, you have increased your population count up to eight. Having a 4-color experiment, you’ve increased your population count to sixteen. And onward it goes until you With this type of intricacy, careful experimental organizing is not a luxury, it’s a prerequisite. Here are 7 methods for preparing and analyzing 10-color flow cytometry experiments. Look at the complete article here - http://expertcytometry.com/flow-cytometry-data-analysis-tips-for-multi-color-experiment Less