Protein and Peptide Characterization in Biopharmaceuticals Molecular mass determination achieves precise molecular weights for protein/peptide drugs. Common protein drugs Molecular Weight include antibodies (intact, deglycosylated, or reduced MW Analysis...
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Protein and Peptide Characterization in Biopharmaceuticals Molecular mass determination achieves precise molecular weights for protein/peptide drugs. Common protein drugs Molecular Weight include antibodies (intact, deglycosylated, or reduced MW Analysis can be measured), growth factors, etc.; Common peptide (MALDI-TOF, LC-MS) drugs include insulin, cabergoline, terlipressin, etc. Peptide profiling of protein/peptide drug molecules is a key element of drug quality control, which is important for theo- retical sequence confirmation, advanced structural analysis, and biological function study. Peptide profiling can confirm the theoretical sequence and modification of protein/peptide. Peptide Mapping 1) Protein sequence confirmation: visualize theoretical se- Analysis quences matching with different abundance, peptide cov- (LC-MS/MS) erage of conventional protein samples can reach up to 100% 2) Peptide mapping analysis: provides all information on amino acid sequences. 3) N/C-terminal ami
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