Cribado de alto rendimiento de CO2

High throughput screening of CO2-tolerating microalgae using GasPak bags Zheng Liu, Fan Zhang and Feng Chen* Abstract Background Microalgae are diverse in terms of their speciation and function. More than 35,000 algal strains have been described, and... More

High throughput screening of CO2-tolerating microalgae using GasPak bags Zheng Liu, Fan Zhang and Feng Chen* Abstract Background Microalgae are diverse in terms of their speciation and function. More than 35,000 algal strains have been described, and thousands of algal cultures are maintained in different culture collection centers. The ability of CO2 uptake by microalgae varies dramatically among algal species. It becomes challenging to select suitable algal candidates that can proliferate under high CO2 concentration from a large collection of algal cultures. Results Here, we described a high throughput screening method to rapidly identify high CO2 affinity microalgae. The system integrates a CO2 mixer, GasPak bags and microplates. Microalgae on the microplates will be cultivated in GasPak bags charged with different CO2 concentrations. Using this method, we identified 17 algal strains whose growth rates were not influenced when the concentration of CO2 was increased from 2 to 20% (v Less