High throughput screening of CO2-tolerating microalgae using GasPak bags
Zheng Liu, Fan Zhang and Feng Chen*
Abstract
Background
Microalgae are diverse in terms of their speciation and function. More than 35,000 algal strains
have been described, and...
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High throughput screening of CO2-tolerating microalgae using GasPak bags
Zheng Liu, Fan Zhang and Feng Chen*
Abstract
Background
Microalgae are diverse in terms of their speciation and function. More than 35,000 algal strains
have been described, and thousands of algal cultures are maintained in different culture collection
centers. The ability of CO2 uptake by microalgae varies dramatically among algal species. It
becomes challenging to select suitable algal candidates that can proliferate under high CO2
concentration from a large collection of algal cultures.
Results
Here, we described a high throughput screening method to rapidly identify high CO2 affinity
microalgae. The system integrates a CO2 mixer, GasPak bags and microplates. Microalgae on the
microplates will be cultivated in GasPak bags charged with different CO2 concentrations. Using this
method, we identified 17 algal strains whose growth rates were not influenced when the
concentration of CO2 was increased from 2 to 20% (v
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